Reproduction Abstracts

Short-term effects of cryopreservation on embryo development, survival and after embryo transfer (ET), resulting pregnancy rate have been documented. However, long-term effects on offspring remain relatively unexplored.Materials and methods: Using a mouse model, we investigated the effect of vitrification on blastocyst trophectoderm (TE) and inner cell mass (ICM) cell numbers by differential staining and postnatal growth and cardiovascular and metabolic ...

Introduction: Maternal depletion of insulin in serum and branched-chain amino acids (BCAA) in uterine luminal fluid occurs in response to dietary protein undernutrition during the periconceptional period which leads to adverse offspring growth and cardiovascular phenotype (e.g. high blood pressure). This study aimed to determine whether insulin and/or BCAA depletion might be inductive in vivo factors for postnatal phenotype using an in vitro embryo culture mo...

Introduction: Maternal low protein diet exclusively during preimplantation mouse development (Emb-LPD) induces compensatory changes in extra-embryonic lineages including primitive endoderm (PE) to enhance nutrient delivery during later gestation, for example, stimulation of endocytosis. Whilst such responses protect fetal growth, adult offspring exhibit increased cardiometabolic disease risk. Here, we investigate epigenetic mechanisms underlying maternal dietary programming, u...

The embryonic environment can induce permanent changes in metabolism and increase disease risk in adults. Rodent models show that modifications in maternal diet exclusively during pre-implantation development can induce metabolic disease in adults. Changes must therefore occur within the early embryo and be maintained throughout development. Determining adaptive mechanisms is challenging due to the size of the early embryo. We generated ES cells from inbred C57BL/6 mice as a m...

Several million babies have been born worldwide since IVF and assisted-reproductive technologies (ART) became available. However, reports link IVF techniques with adverse short- and long-term health outcomes.1,2,3 Using a mouse model, we investigated the effect of IVF on the total cell number in blastocyst stage embryos and the postnatal health of offspring.Methods: Experimental groups; (NM) C57/BL6 non-superovulated females naturally mated wi...

Since the advent of IVF (in vitro fertilisation), several million babies have been born worldwide. However, reports link in vitro techniques with adverse short and long-term health outcomes. Using a mouse model, we investigated the effect of IVF and duration of culture on blastocyst development and cell number and the postnatal health of offspring. Experimental groups (8–13 litters each): NM (natural mating, non-superovulated) IV-ET-2Cell (2-cell embryos...